iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: http://dx.doi.org/10.1038/379131a0
Requirement for Xist in X chromosome inactivation | Nature
Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Requirement for Xist in X chromosome inactivation

Nature volume 379, pages 131–137 (1996)Cite this article

Abstract

The Xist gene has been proposed as a candidate for the X inactivation centre, the master regulatory switch locus that controls X chromosome inactivation. So far this hypothesis has been supported solely by indirect evidence. Here we describe gene targeting of Xist, and provide evidence for its absolute requirement in the process of X chromosome inactivation.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Lyon, M. F. Nature 190, 372–373 (1961).

    Article  ADS  CAS  Google Scholar 

  2. Rastan, S. Curr. Opin. Genet. Dev. 4, 292–297 (1994).

    Article  CAS  Google Scholar 

  3. Rastan, S. & Robertson, E. J. J. Embryol. exp. Morph. 90, 379–388 (1985).

    CAS  PubMed  Google Scholar 

  4. Rastan, S. J. Embryol. exp. Morph. 78, 1–22 (1983).

    CAS  PubMed  Google Scholar 

  5. Cattanach, B. M. & Isaacson, J. H. Genetics 57, 331–346 (1967).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Cattanach, B. M., Pollard, C. E. & Perez, J. N. Genet. Res. 14, 223–235 (1969).

    Article  CAS  Google Scholar 

  7. Cattanach, B. M., Perez, J. N. & Pollard, C. E. Genet. Res. 15, 183–195 (1970).

    Article  CAS  Google Scholar 

  8. Rastan, S. Genet. Res. 40, 139–147 (1982).

    Article  CAS  Google Scholar 

  9. Russell, L. B. & Montgomery, C. S. Genetics 64, 281–312 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Cattanach, B. M. Genet. Res. 23, 291–306 (1974).

    Article  CAS  Google Scholar 

  11. Brown, C. J. & Willard, H. F. Nature 368, 154–156 (1994).

    Article  ADS  CAS  Google Scholar 

  12. Brown, C. J. et al. Nature 349, 82–84 (1991).

    Article  ADS  CAS  Google Scholar 

  13. Brown, C. J. et al. Nature 349, 38–44 (1991).

    Article  ADS  CAS  Google Scholar 

  14. Brockdorff, N. et al. Nature 351, 329–331 (1991).

    Article  ADS  CAS  Google Scholar 

  15. Borsani, G. et al. Nature 351, 325–329 (1991).

    Article  ADS  CAS  Google Scholar 

  16. Brockdorff, N. et al. Cell 71, 515–526 (1992).

    Article  CAS  Google Scholar 

  17. Brown, C. J. et al. Cell 71, 527–542 (1992).

    Article  CAS  Google Scholar 

  18. Kay, G. F. et al. Cell 72, 171–182 (1993).

    Article  CAS  Google Scholar 

  19. Kay, G. F., Barton, S. C., Surani, M. A. & Rastan, S. Cell 77, 639–650 (1994).

    Article  CAS  Google Scholar 

  20. McCarrey, J. R. & Dilworth, D. D. Nature Genet. 2, 200–203 (1992).

    Article  CAS  Google Scholar 

  21. Salido, E. C., Yen, P. H., Mohandas, T. K. & Shapiro, L. J. Nature Genet 2, 196–199 (1992).

    Article  CAS  Google Scholar 

  22. Green, M. C. in Genetic Variants and Strains of the Laboratory Mouse 2nd edn (eds Lyon, M. F.& Searle, A. G.) 282 (Oxford Univ. Press, 1989).

    Google Scholar 

  23. Norris, D. P. et al. Cell 77, 41–51 (1994).

    Article  CAS  Google Scholar 

  24. Singer Sam, J., LeBon, J. M., Dai, A. & Riggs, A. D. PCR Meth. Appl. 1, 160–163 (1992).

    Article  CAS  Google Scholar 

  25. Singer Sam, J., Chapman, V., LeBon, J. M. & Riggs, A. D. Proc. natn. Acad. Sci. U.S.A. 89, 10469–10473 (1992).

    Article  ADS  CAS  Google Scholar 

  26. Buzin, C. H., Mann, J. R. & Singer Sam, J. Development 120, 3529–3536 (1994).

    CAS  PubMed  Google Scholar 

  27. Pamieri, S. L., Peter, W., Hess, H. & Scholer, H. R. Devl Biol. 166, 259–267 (1994).

    Article  Google Scholar 

  28. Mansour, L. S., Thomas, K. R. & Capecchi, M. R. Nature 336, 348–353 (1988).

    Article  ADS  CAS  Google Scholar 

  29. Dutrillaux, B. & Fosse, A-M. Annls Genet. 17, 207–211 (1974).

    CAS  Google Scholar 

  30. Tada, T., Tada, M. & Takagi, N. Development 119, 813–821 (1993).

    CAS  PubMed  Google Scholar 

  31. Takagi, N. & Martin, G. R. Devl Biol. 103, 425–433 (1984).

    Article  CAS  Google Scholar 

  32. Agulnik, A. I. et al. Hum. molec. Genet. 3, 879–884 (1994).

    Article  CAS  Google Scholar 

  33. Wu, J. et al. Nature Genet. 7, 491–496 (1994).

    Article  CAS  Google Scholar 

  34. McMahon, A. & Monk, M. Genet. Res. 41, 69–83 (1983).

    Article  CAS  Google Scholar 

  35. Takagi, N. & Abe, K. Development 109, 189–201 (1990).

    CAS  PubMed  Google Scholar 

  36. Simmler, M. C., Cattanach, B. M., Rasberry, C., Rougeulle, C. & Avner, P. Mamm. Genome 4, 523–530 (1993).

    Article  CAS  Google Scholar 

  37. Robertson, E. J. in Teratocarcinomas and Embryonic Stem Cells, a Practical Approach (ed Robertson, E. J.) 71–112 (IRL, Oxford, 1987).

    Google Scholar 

  38. Scholer, H. R., Rupperts, S., Suzuki, N., Chowdury, K. & Gruss, P. Nature 344, 435–439 (1990).

    Article  ADS  CAS  Google Scholar 

  39. Brockdorff, N., Kay, G., Cattanach, B. M. & Rastan, S. Mamm. Genome 1, 152–157 (1991).

    Article  CAS  Google Scholar 

  40. Ramirez-Solis, S. et al. Analyt. Biochem. 201, 331–335 (1992).

    Article  CAS  Google Scholar 

  41. Zinn, A. R. et al. Genomics 11, 1097–1101 (1991).

    Article  CAS  Google Scholar 

  42. Mardon, G. et al. Molec. cell. Biol. 10, 681–688 (1990).

    Article  CAS  Google Scholar 

  43. Ball, T. C., Hirayama, F. & Ogawa, M. Blood 85, 3086–3092 (1995).

    CAS  PubMed  Google Scholar 

  44. Nagy, A. & Rossant, J. in Gene Targeting, a Practical Approach (ed. Joyner, A. L.) 147–178 (IRL, Oxford, 1993).

    Google Scholar 

  45. Grumbach, M. M., Morishima, A. & Taylor, J. H. Proc. natn. Acad. Sci. U.S.A. 49, 581–589 (1963).

    Article  ADS  CAS  Google Scholar 

  46. Webb, S., de Vries, T. J. & Kaufman, M. H. Genet. Res. 59, 205–214 (1992).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Graham F. Kay

    Present address: Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane, Q4029, Australia

  2. Sohaila Rastan

    Present address: The Medical Research Council, 20 Park Crescent, London, WIN 4AL, UK

  3. Neil Brockdorff: To whom correspondence should be addressed.

Authors and Affiliations

  1. Section of Comparative Biology, MRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London, W12 ONN, UK

    Graeme D. Penny, Graham F. Kay, Steven A. Sheardown, Sohaila Rastan & Neil Brockdorff

Authors
  1. Graeme D. Penny
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Graham F. Kay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Steven A. Sheardown
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sohaila Rastan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Neil Brockdorff
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Penny, G., Kay, G., Sheardown, S. et al. Requirement for Xist in X chromosome inactivation. Nature 379, 131–137 (1996). https://doi.org/10.1038/379131a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/379131a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing